LOCUS       40924_34436        4635 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pPha-DUAL-[2xNR], complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Zauner S, Hempel F.
  TITLE     Inducible shuttle vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Zauner S, Hempel F.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2011) Cell Biology, Philipps-University Marburg, 
            Karl-von-Frisch Str. 8, Marburg 35032, Germany
REFERENCE   3  (bases 1 to 4635)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4635)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-JUN-2011) Cell Biology, Philipps-University Marburg, 
            Karl-von-Frisch Str. 8, Marburg 35032, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      36..451
                     /note="nitrate reductase; derived from Phaeodactylum 
                     tricornutum"
                     /regulatory_class="promoter"
     misc_feature    452..472
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      473..737
                     /note="nitrate reductase; derived from Phaeodactylum 
                     tricornutum"
                     /regulatory_class="terminator"
     regulatory      782..1197
                     /note="nitrate reductase; derived from Phaeodactylum 
                     tricornutum"
                     /regulatory_class="promoter"
     misc_feature    1216..1272
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     regulatory      1273..1537
                     /note="nitrate reductase; derived from Phaeodactylum 
                     tricornutum"
                     /regulatory_class="terminator"
     regulatory      1546..1731
                     /note="fcpB; derived from Phaeodactylum tricornutum"
                     /regulatory_class="promoter"
     CDS             1732..2103
                     /label=BleoR
                     /note="antibiotic-binding protein"
     regulatory      2107..2348
                     /label=fcpA
                     /note="fcpA"
                     /regulatory_class="terminator"
     promoter        complement(2358..2376)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2634..3222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3396..4253)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4254..4358)
                     /label=AmpR promoter
ORIGIN
        1 catatgggca ctagaaggaa ggaagcaaag acgaacggaa gtgactgtaa acgagaagtg
       61 cacgaagcct tttcttgtga cgtcacaaac cgaacagccc tacgtggcgt gcgactttcc
      121 ggcactttgg atatcgttgc cctatatgta ttgggtgtat acatgtcgta ttccaactac
      181 gacagaaaac agtcagttac gggtattcga acttccggca cctgcagcga ggagttttgt
      241 gtccgtcgca catcctccgt gctttcggca cctacgtagg cggcaaactt cctgccttcc
      301 gcccgccttg cggcattccg gctccgggcc agaattgccc gggtgttcac aattttgcct
      361 cctcacgaaa aaacgttcta ctttgtattt tggtcgggtt tcggatcctt ccagcaacca
      421 ttctcattca aagtcaccac ttgtgcgaac gactagtgcg gccgccccgc ggaatttact
      481 ttaagactag cgcaattttt ttcttacatt agatgaacat aaaacgacga tgaggatcac
      541 tgttagggaa acgatgaact acgctgagga cttcctatta ttcgtccttt gatggcgtgg
      601 ttgttctttg gtttgcggtc gagcgcaacg accaaggcct gtaccaaaaa cgaagttgat
      661 aatcagatat ttgagttgta acaagaaact aaagcattat tttagcagta gcacgaatca
      721 ggatctaaaa tgaacgcaat tgcatggttt atgtctaagc tgcgttagaa cttagcatat
      781 gcggaagtga ctgtaaacga gaagtgcacg aagccttttc ttgtgacgtc acaaaccgaa
      841 cagccctacg tggcgtgcga ctttccggca ctttggatat cgttgcccta tatgtattgg
      901 gtgtatacat gtcgtattcc aactacgaca gaaaacagtc agttacgggt attcgaactt
      961 ccggcacctg cagcgaggag ttttgtgtcc gtcgcacatc ctccgtgctt tcggcaccta
     1021 cgtaggcggc aaacttcctg ccttccgccc gccttgcggc attccggctc cgggccagaa
     1081 ttgcccgggt gttcacaatt ttgcctcctc acgaaaaaac gttctacttt gtattttggt
     1141 cgggtttcgg atccttccag caaccattct cattcaaagt caccacttgt gcgaacggaa
     1201 ttcgatatca tcgactaatt cgagctcggt acccggggat cctctagagt cgacctgcag
     1261 gcatgcaagc ttcaatttac tttaagacta gcgcaatttt tttcttacat tagatgaaca
     1321 taaaacgacg atgaggatca ctgttaggga aacgatgaac tacgctgagg acttcctatt
     1381 attcgtcctt tgatggcgtg gttgttcttt ggtttgcggc cgagcgcaac gaccaaggcc
     1441 tgtaccaaaa acgaagttga taatcagata tttgagttgt aacaagaaac taaagcatta
     1501 ttttagcagt agcacggatc aggatctaaa atgaacgcat ccggagagat tcaaaattca
     1561 atctgtttgg acctggataa gacacaagag cgacatcctg acatgaacgc cgtaaacagc
     1621 aaatcctggt tgaacacgta tccttttggg ggcctccgct acgacgctcg ctccagctgg
     1681 ggcttcctta ctatacacag cgcgcatatt tcacggttgc cagatgtcaa gatggccaag
     1741 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     1801 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     1861 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     1921 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     1981 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     2041 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     2101 gactgaacct tccttaaaaa tttaattttc attagttgca gtcactccgc tttggtttca
     2161 cagtcaggaa taacactagc tcgtcttcac catggatgcc aatctcgcct attcatggtg
     2221 tataaaagtt caacatccaa agctagaact tttggaaaga gaaagaatat ccgaataggg
     2281 cacggcgtgc cgtattgttg gagtggacta gcagaaagtg aggaaggcac aggatgagtt
     2341 ttctcgaggc cggtctccct atagtgagtc gtattaattt cgataagcca ggttaacctg
     2401 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     2461 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2521 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2641 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2701 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2821 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3061 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3181 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3241 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3301 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3361 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     3421 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3481 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3541 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3601 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3661 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3721 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     3781 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     3841 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     3901 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     3961 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     4021 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     4081 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4141 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4201 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4261 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4321 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4381 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     4441 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     4501 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     4561 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     4621 gtactgagag tgcac
//
